Cleaning, Disinfection And Pipetting Skills Of Bottle-Top Dispenser
The selection of the appropriate suction head has a great influence on the accuracy of the final dispenser, so it is best to select the suction head used in the initial calibration of the dispenser, that is, the suction head recommended by the manufacturer. In operation, when the lower end of the sleeve handle of the dispenser enters the suction head, the dispenser can be swayed from side to side for 1 to 2 seconds and gently pressed down; if it is a bulk suction head, gently pressing the suction head in the direction of the dispenser with hand, then turning the suction head slightly for 1 to 2 seconds.
Range Control of Bottle-top Dispenser
Generally speaking, the range of consumption of dispensers is 10 ≤ 100% of the maximum range of liquid shifters. For specific liquid shifters, the accuracy of the liquid shifters decreases with the decrease of the liquid shifters. In general, the best range is 30 ≤ 100% of the maximum range of liquid shifters. In the process of adjusting the range, the principle of from large to small are obeyed in order to eliminate the mechanical error of the liquid shifters themselves.
Skills for Volatile Samples and High Viscosity Samples
It is necessary to rinse the liquid two times before moving the liquid, drain the liquid as soon as possible after the liquid absorption is completed, and reduce the loss of liquid volatilization as far as possible. In order to avoid damage to the piston, it is recommended to use a filter core suction head. A high viscosity sample is necessary to move the liquid with the reverse discharge mode, and the residence time of 3 to 5 seconds is required for both suction and drainage.
Skills for Fine and Micro Samples and High Contagious Samples
It is necessary to move liquid with a reverse liquid transfer model. In order to reduce sample loss, a low absorption suction head is recommended. For high contagious samples, cross pollution in liquid transfer process such as contamination of bottle-top dispenser tube can be greatly avoided by using filter core suction head.
Skills for High and Low-Temperature Samples
In order to avoid the influence of temperature, you should never wet suction head before the liquid transfer, meanwhile, each suction head should be replaced, and the liquid should be discharged as soon as possible after liquid absorption is completed.
Internal and External Cleaning Method
Proper cleaning methods can maintain a bottle-top dispenser in good condition as long as possible. The following method should be followed strictly: using wet cloth containing soap. Detergent or 60% isopropanol cleaning solution, then removing external dirt from the dispenser, rinsing with double steamed water and drying; if the sample is polluted by mistake, the lower half of the dispenser needs to be removed and operated according to step 1.
Disinfection and Sterilization Method
All bottle-top dispensers should be sterilized at high temperature and high pressure. The details are as follows: rotating loosening liquid remover, packing sterilization part with the sterilized bag, tin paper or Kraft paper for 20 minutes; after sterilization, cooling the dispenser to room temperature, drying and installing.
Tips for Attention
The sealing ring inside the dispenser is free of maintenance and does not need to be removed, so it does not need to be replaced; when sterilizing, ensure that the temperature does not exceed 121 degree Celsius; do not use any additional disinfectants, cleaners or sodium hypochlorite when sterilizing with high temperature, high pressure or ultraviolet irradiation.